%0 Journal Article %T Scavenging effects of probimane on active oxygen free radicals by electron spin resonance %A Dan Ning %A Li Xiao-jie %A Zhao Bao-lu %A Zhang Tan-mu %A Xin Wen-juan %J Acta Pharmacologica Sinica %D 2016 %B 2016 %9 %! Scavenging effects of probimane on active oxygen free radicals by electron spin resonance %K %X Probimane, dl-1,2-bis (4-morpholine-methyl-3, 5-dioxopiperazin-1-yl) propane, is a new antitumor agent synthesized by Shanghai Institute of Materia Medica, Chinese Academy of Sciences. The scavenging effects of probimane on active oxygen radicals produced in 3 different systems were studied with the ESR spin trapping methods. In Fenton's reaction, probimane remarkably scavenged hydroxyl radicals (.OH) and the rate of scavenging .OH by probimane 0.05 mmol/L was 47%, compared to 5% by vitamin E (VE) and 30% by ascorbic acid (AA). In irradiation riboflavin system, in which superoxide (O2-.) was produced, the agent also had the scavenging effects on O2(-.). The rate of scavenging O2-. by probimane 0.05 mmol/L was 13%, higher than that by VE (7%) but lower than that by AA (90%). In cell system where the active oxygen radicals were produced during the respiratory burst of human neutrophils (Neu) stimulated by TPA (tetradecanoylphorbol acetate), probimane exhibited a dose-dependent scavenging action on the radicals. The rate of the radical scavenging by probimane 0.05 mmol/L was 37%, much higher than that by VE (9%) but lower than that by AA (68%). Probimane had no effect on the rate of oxygen consumption by human Neu, measured with spin probe oxymetry. %U http://www.chinaphar.com/article/view/5596 %V 10 %N 5 %P 443-447 %@ 1745-7254