@article{APS5474,
author = {Hong-zhi Sun and Lin Xu and Bo Zhou and Wei-jin Zang and Shu-fang Wu},
title = {Depletion of insulin receptor substrate 2 reverses oncogenic transformation induced by v-src},
journal = {Acta Pharmacologica Sinica},
volume = {32},
number = {5},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the role of insulin receptor substrate 2 (IRS-2) in oncogenic transformation induced by v-src.
Methods: IRS-2 gene was silenced using small interfering RNAs (siRNAs). Nuclear translocation and interaction of IRS-2 with v-src was determined using subcellular fractionation, confocal microscopy, and immunoprecipitation. The activity of the cyclin D1 promoter and r-DNA promoter was measured with a luciferase assay.
Results: Depletion of IRS-2 inhibited R-/v-src cell growth and reverse the oncogenic transformation. IRS-2 bound to src via its two PI3-K binding sites, which are critical for activities involved in the transformation. Nuclear IRS-2 occupied the cyclin D1 and rDNA promoters. The combination of IRS-2 and v-src increased the activity of the two promoters, especially the rDNA promoter.
Conclusion: Depletion of insulin receptor substrate 2 could reverse oncogenic transformation induced by v-src.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/5474}
}