@article{APS5371,
author = {Xiao-ming SUN and Fu-ben XU and Yu-seng WU and Chen-ren LIN and Chang-yan GUO},
title = {Effect of 2-naphthyl-3- (3,4-dimethoxy) phenyl-propenoic acid on the metabolism of arachidonic acid in rabbit platelets},
journal = {Acta Pharmacologica Sinica},
volume = {11},
number = {1},
year = {2016},
keywords = {},
abstract = {Washed rabbit platelets were incubated with [14C] arachidonic acid (AA sodium salt) after being exposed to 2-naphthyl-3-(3,4-dimethoxy) phenyl-propenoic acid (NM-PA), indomethacin (Ind) and imidazole (Imi) or control solvent. The metabolites of AA in rabbit platelets were separated with the system of chloroform: methanol: acetic acid: water (90:8:1:0.8) by thin layer chromatography and quantitated by liquid scintillation counter. The metabolism of AA was influenced by NMPA dose-dependently in the range of 0.05-0.5 mmol/L. The formation of thromboxane B2 (TXB2) and 12-hydroxy-5,8,10-heptadecatrienoic acid (HHT), the final metabolites in the pathway of cyclooxygenase-thromboxane synthetase, were decreased from 24 +/- 6.7 to 3.2 +/- 1.6% and 10.3 +/- 2.49 to 4.7 +/- 2.8%, respectively. Meanwhile, 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12-HETE), the metabolite in the pathway of lipoxygenase, was increased from 11.9 +/- 1.7 to 34 +/- 5.6%. It is suggested that NMPA blocks the pathway of cyclooxygenase-thromboxane synthetase and then changes the direction of arachidonate metabolism in platelets, the activation of platelets is inhibited in this way.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/5371}
}