TY - JOUR AU - Fang Fang AU - Liu Geng-tao PY - 2016 TI - Protective effects of compound FLZ, a novel synthetic analogue of squamosamide, on β-amyloid-induced rat brain mitochondrial dysfunction in vitro JF - Acta Pharmacologica Sinica; Vol 30, No 5 (May 2009): Acta Pharmacologica Sinica Y2 - 2016 KW - N2 - Aim: The aim of the present study was to assess the effects of N -[2-(4-hydroxyphenyl)ethyl]-2-(2,5-dimethoxyphenyl)-3-(3-methoxy-4-hydroxyphenyl) acrylamide (compound FLZ), a novel synthetic analogue of squamosamide, on the dysfunction of rat brain mitochondria induced by Aβ 25–35 in vitro . Methods: Isolated rat brain mitochondria were incubated with aged Aβ 25–35 for 30 min in the presence and absence of FLZ (1–100 μmol/L). The activities of key mitochondrial enzymes, the production of hydrogen peroxide (H 2 O 2 ) and superoxide anion (O 2 · - ), and the levels of glutathione (GSH) in mitochondria were examined. Mitochondrial swelling and the release of cytochrome c from mitochondria were assessed by biochemical and Western blot methods, respectively. Results: Incubation of mitochondria with aged Aβ 25–35 inhibited the activities of α-ketoglutarate dehydrogenase (α-KGDH), pyruvate dehydrogenase (PDH) and respiratory chain complex IV. It also resulted in increased H 2 O 2 and O 2 · - production, and decreased the GSH level in mitochondria. Furthermore, it induced mitochondrial swelling and cytochrome c release from the mitochondria. The addition of FLZ (100 μmol/L) prior to treatment with Aβ 25–35 significantly prevented these toxic effects of Aβ 25–35 on the mitochondria. Conclusion: FLZ has a protective effect against Aβ 25–35 -induced mitochondrial dysfunction in vitro . UR - http://www.chinaphar.com/article/view/5140