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Protective effects of compound FLZ, a novel synthetic analogue of squamosamide, on β-amyloid-induced rat brain mitochondrial dysfunction in vitro

   
@article{APS5140,
	author = {Fang Fang and Geng-tao Liu},
	title = {Protective effects of compound FLZ, a novel synthetic analogue of squamosamide, on β-amyloid-induced rat brain mitochondrial dysfunction  in vitro },
	journal = {Acta Pharmacologica Sinica},
	volume = {30},
	number = {5},
	year = {2016},
	keywords = {},
	abstract = {Aim: The aim of the present study was to assess the effects of N-[2-(4-hydroxyphenyl)ethyl]-2-(2,5-dimethoxyphenyl)-3-(3-methoxy-4-hydroxyphenyl) acrylamide (compound FLZ), a novel synthetic analogue of squamosamide, on the dysfunction of rat brain mitochondria induced by Aβ25–35 in vitro.
 Methods: Isolated rat brain mitochondria were incubated with aged Aβ25–35 for 30 min in the presence and absence of FLZ (1–100 μmol/L). The activities of key mitochondrial enzymes, the production of hydrogen peroxide (H2O2) and superoxide anion (O2·-), and the levels of glutathione (GSH) in mitochondria were examined. Mitochondrial swelling and the release of cytochrome c from mitochondria were assessed by biochemical and Western blot methods, respectively.
 Results: Incubation of mitochondria with aged Aβ25–35 inhibited the activities of α-ketoglutarate dehydrogenase (α-KGDH), pyruvate dehydrogenase (PDH) and respiratory chain complex IV. It also resulted in increased H2O2 and O2·- production, and decreased the GSH level in mitochondria. Furthermore, it induced mitochondrial swelling and cytochrome c release from the mitochondria. The addition of FLZ (100 μmol/L) prior to treatment with Aβ25–35 significantly prevented these toxic effects of Aβ25–35 on the mitochondria.
 Conclusion: FLZ has a protective effect against Aβ25–35-induced mitochondrial dysfunction in vitro.},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/5140}
}