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PKC promotes proliferation of airway smooth muscle cells by regulating cyclinD1 expression in asthmatic rats

   
@article{APS5094,
	author = {Li-fen Qiao and Yong-jian Xu and Xian-sheng Liu and Jun-gang Xie and Jin Wang and Chun-ling Du and Jian Zhang and Wang Ni and Shi-xin Chen},
	title = {PKC promotes proliferation of airway smooth muscle cells by regulating cyclinD1 expression in asthmatic rats},
	journal = {Acta Pharmacologica Sinica},
	volume = {29},
	number = {6},
	year = {2016},
	keywords = {},
	abstract = {Aim: To determine whether protein kinase C (PKC) has any effect on the expression of cyclinD1, a key regulator of growth control and G1/S transition, and to investigate the underlying molecular mechanisms of PKC involving the remodeling of the asthmatic airway smooth muscle (ASM).
 Methods: The treatment of synchronized ASM cells from asthmatic rats with PKC-specific agonist phorbol 12-myristate 13-acetate (PMA) and antagonist 2-\{1-[3-(amidinothio) propyl]-1H-indol-3-yl\}-3-(1-methylindol-3-yl) maleimide methanesulfonate salt (Ro31-8220) was followed by the proliferation assay. PKCα and cyclinD1 expressions in ASM cells (ASMC) were detected by RT-PCR and Western blotting. The relation between PKCαand cyclinD1 was assessed by linear regression analysis. The effect of the construct recombinant plasmid pcDNA3.1-antisense cyclinD1 (pcDNA3.1-ascyclinD1) on the proliferation of ASMC was found to be induced by PMA.
 Results: The data showed phorbol ester-dependent PKCα promoted the proliferation of ASMC. The closely-positive correlation existed between the expression of PKCα and cyclinD1 at the transcriptional (r=0.821, P},
	issn = {1745-7254},	url = {http://www.chinaphar.com/article/view/5094}
}