@article{APS5093,
author = {Hong-ling Wei and Chun-yu Zhang and Hong-fang Jin and Chao-shu Tang and Jun-bao Du},
title = {Hydrogen sulfide regulates lung tissue-oxidized glutathione and total antioxidant capacity in hypoxic pulmonary hypertensive rats},
journal = {Acta Pharmacologica Sinica},
volume = {29},
number = {6},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the modulatory effect of sudium hydrosulfide on lung tissue-oxidized glutathione and total antioxidant capacity in the development of hypoxic pulmonary hypertension (HPH).
Methods: After 21 d of hypoxia, the mean pulmonary artery pressure was measured by cardiac catheterization. The plasma H2S level and production of H2S in the lung tissues were determined by using a spectrophotometer. The lung homogenates were assayed for total antioxidant capacity (T-AOC), superoxide dismutase (SOD), oxidized glutathione (GSSG), reduced glutathione and malonaldehyde by colorimetry. The mRNA level of SOD was analyzed by real-time PCR, and the SOD expression was detected by Western blotting.
Results: In the hypoxia group, the plasma H2S concentration and H2S production in the lung was significantly decreased compared with the control group (187.2±13.1 vs 299.6±12.4 μmol/L; 0.138±0.013 vs 0.289±0.036 nmol·mg−1·min−1, P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/5093}
}