%0 Journal Article %T Bufalin promotes the release of noradrenaline from isolated guinea pig vas deferens %A HAN Yong-jing %A JIN Zhu-hua %A HOU Qing-chang %A CUI Rong-fen %A SONG Han-ying %J Acta Pharmacologica Sinica %D 2016 %B 2016 %9 %! Bufalin promotes the release of noradrenaline from isolated guinea pig vas deferens %K %X Bufalin (B), one of the active principles extracted from toad venom, caused a long-lasting contraction of isolated vas deferens of guinea pig at the concentrations of 1.3-9.1 microM. The B-induced contraction was blocked or even abolished by a pretreatment with phentolamine 10 microM, reserpine 2 (mg/kg) d*2 d, verapamil 1 microM or stored at 4 degrees for 7 d, and was partly inhibited by a pretreatment with bretylium 0.73 mM or cocaine 15 microM, but unaffected by atropine 0.43 microM or cyproheptadine 1 microM. When the vas deferens was incubated with B, a marked release of NA was observed. This response was prevented by reserpine. These results suggested that the B-induced contraction of vas deferens might be due to NA released from the adrenergic nerve terminals. %U http://www.chinaphar.com/article/view/5023 %V 7 %N 4 %P 314-317 %@ 1745-7254