@article{APS4862,
author = {Luciana Ferreira Leite and Renato Santiago Gomez and Matheus de Castro Fonseca and Marcus Vinicius Gomez and Cristina Guatimosim},
title = {Effect of intravenous anesthetic propofol on synaptic vesicle exocytosis at the frog neuromuscular junction},
journal = {Acta Pharmacologica Sinica},
volume = {32},
number = {1},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the presynaptic effects of propofol, a short-acting intravenous anesthetic, in the frog neuromuscular junction.
Methods: Frog cutaneous pectoris nerve muscle preparations were prepared. A fluorescent tool (FM1-43) was used to visualize the effect of propofol on synaptic vesicle exocytosos in the frog neuromuscular junction.
Results: Low concentrations of propofol, ranging from 10 to 25 μmol/L, enhanced spontaneous vesicle exocytosis monitored by FM1-43 in a Ca2+-dependent and Na+-independent fashion. Higher concentrations of propofol (50, 100, and 200 μmol/L) had no effect on spontaneous exocytosis. By contrast, higher concentrations of propofol inhibited the Na+-dependent exocytosis evoked by 4-aminopyridine but did not affect the Na+-independent exocytosis evoked by KCl. This action was similar and non-additive with that observed by tetrodotoxin, a Na+ channel blocker.
Conclusion: Our data suggest that propofol has a dose-dependent presynaptic effect at the neuromuscular transmission which may help to understand some of the clinical effects of this agent on neuromuscular function.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/4862}
}