TY - JOUR AU - Yang Jin-long AU - Shen Zu-ming AU - Sun Yi-fang AU - Han Jia-xian AU - Xu Bin PY - 2016 TI - Cultured human hepatoma cells (BEL-7404) for anticancer drugs screening JF - Acta Pharmacologica Sinica; Vol 6, No 2 (June 1985): Acta Pharmacologica Sinica Y2 - 2016 KW - N2 - A quantitative method of tumor cell culture for evaluating anticancer agents in viro was established. Typical cell-growth curve was seen when human hepatoma( BEL-7404) 2.5*10(2)-2.5*10(4) cell/well were placed in plastic microtitre plates. The amount of radioactivity of [3H]leucine incorporated into cells was employed to assay the cell-growth rate. 10-Hydroxycamptothecin 1-100 microg/mL inhbited significantly the growth of BEL-7404. The incorporation of [3H]leucine into the protein of tumor cells was inhibited by 31-71%. Among 40 drugs tested,harringtonine, homoharringtonine, and nevadensin inhibited markedly the growth of BEL-7404 cells. UR - http://www.chinaphar.com/article/view/4785