@article{APS4721,
author = {Yi-jun Fan and Jun Zhou and Mao Li},
title = {Effect of maytansine on the cell cycle of Ehrlich ascites carcinoma cells in mice},
journal = {Acta Pharmacologica Sinica},
volume = {6},
number = {1},
year = {2016},
keywords = {},
abstract = {The effect of maytansine on the cell cycle of Ehrlich ascites carcinoma(EAC) cells in mice was studied by autoradiography Maytansine 0.1 mg/kg was injected ip on d 4 after ip inoculation of 10(7) cells, the metaphase labeling curve showed a prolongation of T(G2+M).The [3H] TdR pulse labeling index( LI) was markedly reduced in 8 h; but the effect of maytansine on the mitotic index was contrary to the pulse LI. However, the anaphase and telophase cells were not noticed in 6 h. The [3H]TdR continuous LI in 12 h was 96% in the untreated cells, but 81% at the same time and 96% at the 24th in the treated cells. The grain counts of EAC cells labeled by [3H]TdR and [3H]UR did not decrease. These results showed that maytansine was extremely effective in producing mitotic arrest of the EAC cells, and the cells blocked in M phase could partial-synchronously enter S phase. LI curve of [3H]TdR continuous label for the cells on d 11 of tumor growth indicated that EAC cells in Go phase could not re-enter the cell cycle after ip maytansine.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/4721}
}