@article{APS4688,
author = {Shu-xu Du and Hong-fang Jin and Ding-fang Bu and Xia Zhao and Bin Geng and Chao-shu Tang and Jun-bao Du},
title = {Endogenously generated sulfur dioxide and its vasorelaxant effect in rats},
journal = {Acta Pharmacologica Sinica},
volume = {29},
number = {8},
year = {2016},
keywords = {},
abstract = {Aim: The present study was designed to explore the endogenous production and localization of the sulfur dioxide (SO2)/aspartate aminotransferase pathway in vascular tissues of rats and to examine its vasorelaxant effect on isolated aortic rings, as well as the possible mechanisms.
Methods: The content of SO2 in the samples was determined by using high performance liquid chromatography with fluorescence detection. Aspartate aminotransferase activity and its gene expression were measured by an enzymatic method and quantitative RT–PCR, respectively. Aspartate aminotransferase mRNA location in aorta was detected by in situ hybridization. The vasorelaxant effect of SO2 on isolated aortic rings of the rats was investigated in vitro. L-type calcium channel blocker, nicardipine, and L-type calcium channel agonist, Bay K8644, were used to explore the mechanisms by which SO2 relaxed the aortic rings.
Results: Aorta had the highest SO2 content among the vascular tissues tested (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/4688}
}