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Effects of notoginosides on proliferation and upregulation of GR nuclear transcription factor in hematopoietic cells

  
@article{APS4684,
	author = {Rui-lan Gao and Xiao-hong Chen and Xiao-jie Lin and Xu-dai Qian and Wei-hong Xu and Beng Hock Chong},
	title = {Effects of notoginosides on proliferation and upregulation of GR nuclear transcription factor in hematopoietic cells},
	journal = {Acta Pharmacologica Sinica},
	volume = {28},
	number = {5},
	year = {2016},
	keywords = {},
	abstract = {Aim: To investigate the effects of panax notoginosides (PNS) on the proliferation of human hematopoietic stem/progenitor cells, and to explore the signaling pathway of the nuclear transcription factor of the glucocorticoid receptor (GR-NTF) initiated by PNS related with the proliferation.
Methods: 
The human CD34+ cells and bone marrow nuclear cells were exposed to PNS at a concentration of 0, 10, 25, 50, and 100 mg/L, respectively, in semi-solid culture system to observe colony forming unite of all lineages, granulocyte, erythrocyte, and megakaryocyte (CFU-GEMM, CFU-GM, CFU-E, and CFU-MK). Three lineages of human hematopoietic cell lines, including granulocytic HL-60, erythrocytic K562, megakaryocytic CHRF-288, and Meg-01 cells were incubated with PNS at 20 mg/L for 14 d. Meanwhile, dexamethasone (Dex) was used as a positive control. The nuclear protein of the cells was analyzed by Western blotting with monoclonal antibodies against the amino or carboxyl terminus of GR-NTF. Electrophoretic mobility shift assay performed by using the 32P-radiolabeled GR-NTF consensus oligonucleotide.
Results: PNS promoted the proliferation of CD34+ cells and significantly raised the colony numbers of CFU-GEMM by 34.7%±16.0% over the non-PNS control (P},
	url = {http://www.chinaphar.com/article/view/4684}
}