@article{APS4678,
author = {Min Fu and Zhao-hui Sun and Min Zong and Xiang-ping He and Huan-cong Zuo and Zuo-ping Xie},
title = { Deoxyschisandrin modulates synchronized Ca 2+ oscillations and spontaneous synaptic transmission of cultured hippocampal neurons},
journal = {Acta Pharmacologica Sinica},
volume = {29},
number = {8},
year = {2016},
keywords = {},
abstract = {Aim: Deoxyschisandrin is one of the most effective composites of Schisandra chinensis, a famous Chinese medicine widely used as an antistress, anti-aging, and neurological performance-improving herb. In this study, we examined its specific mechanisms of action on cultured hippocampal neurons.
Methods: Hippocampal neurons, primarily cultured for 9–11 d in vitro, were used for this study. DS were dissolved in DMSO and applied to calcium imaging and whole-cell patch clamp.
Results: The application of 3 mg/L DS decreased the frequency of spontaneous and synchronous oscillations of intracellular Ca2+ to 72%±2% (mean±SEM), and the spontaneous inhibitory postsynaptic currents to 60%±3% (mean±SEM). The inhibitory concentraton 50% (IC50) for the effect of DS on calcium oscillations was 3.8 mg/L. DS also depressed the high voltage-gated Ca2+ channel and the voltage-gated Na+ channel currents at the same time point. It had no effect, however, on voltage-gated K+ and spontaneous excitatory postsynaptic currents.
Conclusion: DS inhibited the spontaneous and synchronous oscillations of intracellular Ca2+ through the depression of influx of extracellular calcium and the initiation of action potential. By repressing the spontaneous neurotransmitter release, DS modulated the neuronal network activities.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/4678}
}