%0 Journal Article
%T Effect of betulinic acid on the regulation of Hiwi and cyclin B1 in human gastric adenocarcinoma AGS cells
%A Yang Li-jing
%A Chen Yan
%A Ma Qi
%A Fang Jun
%A He Jing
%A Cheng Yi-quan
%A Wu Qiu-ling
%J Acta Pharmacologica Sinica
%D 2016
%B 2016
%9 
%! Effect of betulinic acid on the regulation of Hiwi and cyclin B1 in human gastric adenocarcinoma AGS cells
%K 
%X  Aim:  To investigate the effect of betulinic acid (BA) on the proliferation, apoptosis and cell cycle of gastric adenocarcinoma cell AGS  in vitro  and elucidate the underlying mechanisms.   Methods:  The effect of BA on the proliferation of AGS cells was measured by using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2 H -tetrazolium (MTT) assay. Apoptosis was analyzed by using Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) double-labeled flow cytometry (FCM) and Hoechst 33258 staining. The influence of BA on cell cycle of AGS cells was tested by PI staining. Both FCM and reverse transcription-PCR (RT-PCR) technologies were applied to detect the expression of Hiwi and Cyclin B1.   Results:  BA exhibited significant cell proliferation inhibition, as well as its potency of inducing apoptosis in AGS cells  in vitro  in a timeand dose-dependent manner. The IC 50  value for 24 h was 18.25 μg/mL (95% confidence interval: 15.16 to 27.31 μg/mL). Cells treated with BA showed increased cell population in G 2 /M phase, with decreased S phase population. The expression of Hiwi and Cyclin B1 was down-regulated in BA-treated AGS cells in a dose-dependent manner.   Conclusion:  BA exerted potent effect on growth inhibition, G 2 /M cell cycle arrest and induction of apoptosis in AGS cells  in vitro , possibly associated with the down-regulation of Hiwi and its downstream target Cyclin B1 expression. The potent antitumor capacity of BA suggested that it could be a promising new experimental anticancer agent in human gastric adenocarcinoma treatment.
%U http://www.chinaphar.com/article/view/4509
%V 31
%N 1
%P 66-72
%@ 1745-7254