TY - JOUR AU - Rosta Klara AU - Tulassay Eszter AU - Enzsoly Anna AU - Ronai Katalin AU - Szantho Ambrus AU - Pandics Tamas AU - Fekete Andrea AU - Mandl Peter AU - Ver Agota PY - 2016 TI - Insulin induced translocation of Na + /K + -ATPase is decreased in the heart of streptozotocin diabetic rats JF - Acta Pharmacologica Sinica; Vol 30, No 12 (December 2009): Acta Pharmacologica Sinica Y2 - 2016 KW - N2 - Aim: To investigate the effect of acute insulin administration on the subcellular localization of Na + /K + -ATPase isoforms in cardiac muscle of healthy and streptozotocin-induced diabetic rats. Methods: Membrane fractions were isolated with subcellular fractionation and with cell surface biotinylation technique. Na + /K + -ATPase subunit isoforms were analysed with ouabain binding assay and Western blotting. Enzyme activity was measured using 3- O -methylfluorescein-phosphatase activity. Results: In control rat heart muscle α1 isoform of Na + /K + ATPase resides mainly in the plasma membrane fraction, while α2 isoform in the intracellular membrane pool. Diabetes decreased the abundance of α1 isoform (25 %, P Conclusion: This study demonstrate that insulin influences the plasma membrane localization of Na + /K + -ATPase isoforms in the heart. α2 isoform translocation is the most vulnerable to the reduced insulin response in diabetes. α1 isoform also translocates in response to insulin treatment in healthy rat. Insulin mediates Na + /K + -ATPase α1- and α2-subunit translocation to the cardiac muscle plasma membrane via a PI3-kinase-dependent mechanism. UR - http://www.chinaphar.com/article/view/4451