@article{APS4378,
author = {Li-cheng Dai and Xiang Wang and Xing Yao and Li-shan Min and Jin-liang Ping and Jian-fang He},
title = {Antisense oligonucleotides targeting midkine inhibit tumor growth in an in situ human hepatocellular carcinoma model},
journal = {Acta Pharmacologica Sinica},
volume = {28},
number = {3},
year = {2016},
keywords = {},
abstract = {Aim: To evaluate the in vivo antitumor effects of antisense oligonucleotides targeting midkine (MK-AS). Methods: An in situ human hepatocellular carcinoma (HCC) model was established in mice livers orthotopically. The MK-AS and 5-fluorouracil (5-Fu) were administered intravenously. The tumor sizes and plasma alpha-fetoprotein (AFP) were measured by calipers and radiation immunoassay respectively. The morphology of tumors was evaluated by hematoxylin-eosin staining of histological sections. Human MK, p53, Bax, Bcl-2, and caspase-3 protein content were detected by Western blotting. Results: MK-AS significantly inhibited in situ human HCC growth in mice compared with the saline group in a dose-dependent manner. After the treatment with MK-AS or with 5-Fu, the plasma AFP concentration decreased in a dose-dependent manner. Interestingly, MK-AS also clearly downregulated the protein level of Bcl-2, and upregulated p53, Bax, and caspase-3 in the hepatocellular carcinoma tissue. Conclusion: These results demonstrated that MK-AS was an effective antitumor antisense oligonucleotide in vivo in mice; its antitumor effect is associated with the increase of pro-apoptotic proteins, such as p53, Bax, and caspase-3, and the decrease of the anti-apoptotic protein, Bcl-2.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/4378}
}