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Effect of TGF-β/Smad signaling pathway on lung myofibroblast differentiation

	author = {Li Gu and Yuan-jue Zhu and Xiao Yang and Zi-jian Guo and Wen-bing Xu and Xin-lun Tian},
	title = {Effect of TGF-β/Smad signaling pathway on lung myofibroblast differentiation},
	journal = {Acta Pharmacologica Sinica},
	volume = {28},
	number = {3},
	year = {2016},
	keywords = {},
	abstract = {Aim:  Myofibroblasts play important roles in the pathogenesis of lung fibrosis. Transforming growth factor (TGF)-β1 has been widely recognized as a key fibrogenic cytokine. The major signaling pathway of TGF-β1 is through cytoplasmic Smad proteins. Our study investigated the role of individual TGF-β1/Smad signal proteins in mediating α-smooth muscle actin (α-SMA) gene expression, which is a well-known key marker of myofibroblast differentiation. Methods:  We transiently cotransfected α-SMA promoter-luciferase fusion plasmid (p895-Luc) and Smad expression plasmids and measured Luc activity in TGF-β1-treated human fetal lung fibroblasts. We induced Smad3 knockout mice lung fibrosis by bleomycin. α-SMA protein expression was assessed by Western blotting. Collagen protein was analyzed by measuring hydroxyprolin. Myofibroblast morphology was assessed by immunohistochemistry. Results:  We found that the overexpression of Smad3, not Smad2 markedly increased TGF-β1-induced α-SMA promoter activity and α-SMA protein expression in vitro, whereas the overexpression of dominant negative mutant Smad3 and Smad7 repressed TGF-β1-induced α-SMA gene expression. Compared to wild-type mice, Smad3 knockout mice showed attenuated lung fibrosis after bleomycin treatment, manifested by lower collagen production and myofibroblast differentiation. Conclusion:  Our study suggested TGF-β1/Smad3 is a major pathway which regulated the myofibroblast differentiation. This result indicates a potential significance for future attempts of attenuating the progression of human lung fibrosis by the inhibition of the Smad3 cascade.},
	issn = {1745-7254},	url = {}