@article{APS4246,
author = {Fang Fang and Geng-tao Liu},
title = {Novel squamosamide derivative (compound FLZ) attenuates Aβ 25–35 - induced toxicity in SH-SY5Y cells},
journal = {Acta Pharmacologica Sinica},
volume = {29},
number = {2},
year = {2016},
keywords = {},
abstract = {Aim: The aim of the present study was to investigate the protective effect of compound N-[2-(4-hydroxy-phenyl)-ethyl]-2-(2,5-dimethoxy-phenyl)-3-(3-methoxy-4-hydroxy-phenyl)-acrylamide (compound FLZ), a novel synthetic analogue of nature squamosamide, on Aβ25–35-induced toxicity and its active mechanism in human neuroblastoma SH-SY5Y cells.
Methods: SH-SY5Y cells were preincubated with various concentrations of compound FLZ for 30 min and then cultivated with Aβ25–35 (25 μmol/L) for 48 h to induce neurotoxicity. Cell viability, lactate dehydrogenase (LDH) release, and the glutathione (GSH) level were determined by a biochemical analysis. The cell apoptotic ratio and intracellular reactive oxygen species (ROS) level were measured by a flow cytometry analysis. The expression of apoptosis protein (Bcl-2 and Bax) and cytochrome c release were assayed by the Western blot method.
Results: The pretreatment of SH-SY5Y cells with FLZ (1 and 10 μmol/L) markedly increased cell viability and decreased LDH release and morphological injury. Also, FLZ attenuated the Aβ25–35-induced apoptotic cell ratio, regulated the apoptosis protein (Bcl-2 and Bax) expression, and decreased the cytochrome c release from mitochondria. FLZ also significantly inhibited the generation of ROS and the depletion of GSH induced by Aβ25–35 in SH-SY5Y cells.
Conclusion: FLZ has protective action against Aβ25–35-induced toxicity in SH-SY5Y cells, which might be mediated through its antioxidant property.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/4246}
}