@article{APS4196,
author = {Nan Gu and Shu-ping Han and Li Fei and Xiao-qin Pan and Mei Guo and Rong-hua Chen and Xi-rong Guo},
title = {Resistin-binding peptide antagonizes role of resistin on white adipose tissue},
journal = {Acta Pharmacologica Sinica},
volume = {28},
number = {2},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the direct effects of resistin and resistin-binding peptide (RBP) on lipid metabolism and endocrine function in adipose tissue.
Methods: Rat white adipose tissue was cultured in vitro and incubated for 24 h with 30 ng/mL recombinant rat resistin protein (rResistin) or combined with RBP of varying concentrations (1×10-12 mol/L, 1×10-10 mol/L, 1×10-8 mol/L). Free fatty acids (FFA) released into medium was measured by a colorimetric kit. The levels of protein secretion and mRNA expression of TNF-α and adiponectin were detected by ELISA kit and RT-PCR respectively.
Results: The levels of FFA released into medium were significantly increased after 24 h of exposure to rResistin, but significantly decreased after RBP was applied, although there was no difference between the 3 concentrations. The protein level and gene expression of TNF-α in adipose tissue were significantly increased after 24 h of exposure to rResistin, but only obviously decreased after incubated with 1×10-8 mol/L RBP. The levels of protein secretion
and mRNA expression of adiponectin in adipose tissue were significantly decreased after 24 h of exposure to rResistin, but increased after incubated with RBP with the higher concentrations.
Conclusion: RBP can effectively antagonize the role of resistin on the lipid metabolism and endocrine function of adipose tissue.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/4196}
}