@article{APS4021,
author = {Qian Ge and Yong-ming Dong and Zhi-tao Hu and Zheng-xing Wu and Tao Xu},
title = {Characteristics of Ca 2+ -exocytosis coupling in isolated mouse pancreatic bold beta cells},
journal = {Acta Pharmacologica Sinica},
volume = {27},
number = {7},
year = {2016},
keywords = {},
abstract = {Aim: To characterize Ca2+-stimulated exocytosis in isolated mouse pancreatic β cells.
Methods: An improved method was described for isolation of mouse pancreatic β cells by collagenase P. The Ca2+ channel current and the membrane capacitance were examined by using the whole-cell patch clamp recording technique.
Results: Using depolarization and flash photolysis of caged Ca2+ to induce Ca2+-dependent exocytosis in β cell from KM mouse, we have explored the characteristics of the Ca2+ channel current and the relationship between Ca2+ signals and exocytosis. The averaged peak Ca2+ current measured at +20 mV was -60plusminus6 pA (n=13).
Conclusion: We characterized three kinetically different pools of vesicles in mouse pancreatic β cells, namely an immediately releasable pool, a readily releasable pool, and a reserve pool.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/4021}
}