@article{APS3933,
author = {Kai Li and Bin Wan and Zhen-lin Hu and Ying He and Xiao-wen He and Lei Jiang and Shu-han Sun},
title = {Effects of CpG-ODN on gene expression in formation of foam cells},
journal = {Acta Pharmacologica Sinica},
volume = {26},
number = {11},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the effects of CpG-oligodeoxynucleotide (CpG-ODN) on the formation of macrophage foam cells and related gene expression.
Methods: A gene expression profile was examined by microarray techniques, and mRNA expression was detected by reverse transcriptase polymerase chain reaction (RT-PCR). The cholesterol and cholesteryl ester contents of cells were determined by high performance liquid chromatography.
Results: CD36, LPL, and Fcgamma2b, which were related to lipid metabolism and the formation of macrophage foam cells, were upregulated after CpG-ODN stimulation. The mRNA expression related to the formation of foam cells was confirmed by semiquantitative RT-PCR. Moreover, histochemical analysis confirmed that lipid deposits inside cells increased after CpG-ODN treatment. However, using flow cytometry, we found that CpG-ODN had no effect on the expression of membrane receptors.
Conclusion: CpG-ODN up-regulated the expression of genes in macrophage foam cell formation.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3933}
}