%0 Journal Article %T Effects of propyl gallate on interaction between TNF-alpha and sTNFR-I using an affinity biosensor %A Li Jing %A Huang Jia-dong %A Wu Bao-yan %A Chen Qiang %J Acta Pharmacologica Sinica %D 2016 %B 2016 %9 %! Effects of propyl gallate on interaction between TNF-alpha and sTNFR-I using an affinity biosensor %K %X Aim: To study the effects of propyl gallate on the interaction of tumor necrosis factor-alpha (TNF-alpha) with its soluble receptor, sTNFR-I. Methods: Interactions between TNF-alpha and sTNFR-I were analyzed using an IAsys biosensor. sTNFR-I was immobilized on the carboxymethyl dextran (CMD) surface of the IAsys biosensor cuvettes, and TNF-alpha preincubated with different concentrations of propyl gallate was added to the cuvettes. The resonant angle shift caused by the binding between TNF-alpha and sTNFR-I was then recorded. Results: sTNFR-I was immobilized on the CMD surface at a density of 2.76 ng/mm 2 . TNF-alpha then bound the immobilized sTNFR-I specifically, and propyl gallate was able to enhance the binding between TNF-alpha and sTNFR-I in a dose-dependent manner. Conclusion: The binding between TNF-alpha and sTNFR-I is one of the targets that propyl gallate can act on in vivo . The IAsys biosensor offers a new clue as to the study on the mechanisms of action of propyl gallate. %U http://www.chinaphar.com/article/view/3887 %V 26 %N 10 %P 1212-1216 %@ 1745-7254