TY  - JOUR
AU  - Kim So-hyung
AU  - Kang Kyoung-ah
AU  - Zhang Rui
AU  - Piao Mei-jing
AU  - Ko Dong-ok
AU  - Wang Zhi-hong
AU  - Chae Sung-wook
AU  - Kang Sam-sik
AU  - Lee Keun-hwa
AU  - Kang Hee-kyoung
AU  - Kang Hyun-wook
AU  - Hyun Jin-won
PY  - 2016
TI  - Protective effect of esculetin against oxidative stress-induced cell damage via scavenging reactive oxygen species
JF  - Acta Pharmacologica Sinica; Vol 29, No 11 (November 2008): Acta Pharmacologica Sinica
Y2  - 2016
KW  - 
N2  - Aim: To investigate the anti-oxidant properties of esculetin (6,7-dihydro-xycoumarin) against H2O2-induced Chinese hamster lung fibroblast (V79-4) damage.  Methods: The radical scavenging activity was assessed by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical, and intracellular reactive oxygen species (ROS). In addition, lipid peroxidation was assayed by the measure of related substances which react with thiobarbituric acid. The amount of carbonyl formation in protein was determined using a protein carbonyl ELISA kit. As well, cellular DNA damage was detected by Western blot and immuno-fluorescence image. Cell viability was assessed by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide assay.  Results: Esculetin exhibited DPPH radical scavenging, hydroxyl radical scavenging, and intracellular ROS scavenging activities. The radical scavenging activity of esculetin resulted in the protection of cells from lipid peroxidation, protein carbonyl, and DNA damage induced by H2O2. Therefore, esculetin recovered cell viability exposed to H2O2.  Conclusion: Esculetin efficiently attenuated the oxidative stress induced cell damage via its anti-oxidant properties. As a result, esculetin may be useful in the development of functional food and raw materials of medicine.
UR  - http://www.chinaphar.com/article/view/3853