TY  - JOUR
AU  - Li Xiang-hui
AU  - Zhang Jin-chao
AU  - Sui Sen-fang
AU  - Yang Meng-su
PY  - 2016
TI  - Effect of daidzin, genistin, and glycitin on osteogenic and adipogenic differentiation of bone marrow stromal cells and adipocytic transdifferentiation of osteoblasts
JF  - Acta Pharmacologica Sinica; Vol 26, No 9 (September 2005): Acta Pharmacologica Sinica
Y2  - 2016
KW  - 
N2  - Aim: To examine the effect of daidzin, genistin, and glycitin on the osteogenic and adipogenic differentiation of bone marrow stromal cells (MSC) and the adipogenic transdifferentiation of osteoblasts.  Methods: MTT test, alkaline phosphatase (ALP) activity measurement, Oil Red O stain and measurement were employed.  Results: Daidzin, genistin, and glycitin 1times10-8, 5times10-7, 1times10-6, 5times10-6, and 1times10-5 mol/L all promoted the proliferation of primary mouse bone MSC and osteoblasts. Daidzin 5times10-7 mol/L and genistin 1times10-6 mol/L promoted the osteogenesis of MSC. Genistin 1times10-8, 5times10-7, 1times10-6, 5times10-6, and 1times10-5 mol/L and glycitin 1times10-8, 1times10-6, and 1times10-5 mol/L inhibited the adipogenesis of MSC. Daidzin, genistin, and glycitin 1times10-8, 5times10-7, 1times10-6, 5times10-6, and 1times10-5 mol/L all inhibited the adipocytic transdifferentiation of osteoblasts.  Conclusions: Daidzin, genistin, and glycitin may modulate differentiation of MSC to cause a lineage shift toward the osteoblast and away from the adipocytes, and could inhibit adipocytic transdifferentiation of osteoblasts. They could also be helpful in preventing the development of osteonecrosis.
UR  - http://www.chinaphar.com/article/view/3842