%0 Journal Article
%T Immune tolerance induced by adoptive transfer of dendritic cells in an insulin-dependent diabetes mellitus murine model
%A Zhang Cheng-liang
%A Zou Xiao-lei
%A Peng Jia-bei
%A Xiang Ming
%J Acta Pharmacologica Sinica
%D 2016
%B 2016
%9 
%! Immune tolerance induced by adoptive transfer of dendritic cells in an insulin-dependent diabetes mellitus murine model
%K 
%X Aim: To investigate the effect and underlying mechanisms of immune-tolerance  induced by the adoptive transfer of bone marrow (BM)-derived dendritic cells  (DC) in insulin-dependent diabetes mellitus (IDDM) mice. Methods: The IDDM  model was established by a low dose of streptozotocin (STZ) in Balb/c mice. Two  DC subpopulations were generated from the BM cells with granulocyte-macrophage  colony-stimulating factor with or without interleukin-4. The purity and the T  cell stimulatory capability of DC were identified. These cells were used to modulate  autoimmune response in pre-diabetic mice. Blood glucose was examined  weekly; pancreas tissues were taken for histopathological analysis, and CD4+ T  cells were isolated to detect lymphocyte proliferation by MTT assay and the ratio  of CD4+CD25+ T cells by fluorescence-activated cell sorting (FACS). The cytokine  secretion was determined by ELISA analysis. Results: Two DC subsets were  generated from BM, which have phenotypes of mature DC (mDC) and immature  DC (iDC), respectively. The level of blood glucose decreased significantly by  transferring iDC (P
%U http://www.chinaphar.com/article/view/3810
%V 28
%N 1
%P 98-104
%@ 1745-7254