@article{APS3705,
author = {Wei-na Wang and Xin-bo Yang and He-zhi Liu and Zheng-ming Huang and Guang-xia Wu},
title = {Effect of Oenanthe javanica flavone on human and duck hepatitis B virus infection},
journal = {Acta Pharmacologica Sinica},
volume = {26},
number = {5},
year = {2016},
keywords = {},
abstract = {Aim: To study the antiviral effect of Oenanthe javanica flavones (OjF) on human hepatoma HepG2.2.15 culture system and duck hepatitis B virus (DHBV) infection.
Methods: (1) After incubation for 24 h, the 2.2.15 cells were treated with different concentrations of OjF for 12 d. The cell alteration was observed by microscope. The presence of HBsAg and HBeAg were measured using the enzyme immunoassay kit after 2.2.15 cells were treated with OjF for 9 d. (2) Ducklings infected with DHBV intravenously were divided into 5 groups and treated with OjF, acyclovir (ACV), and normal saline respectively for 10 d. All the ducklings were bled before, during, and after treatments at different times, and serum levels of DHBV-DNA were detected by a dot-blot hybridization assay.
Results: (1) The 50% toxic concentration (TC50) of OjF was 2.28 g/L. The maximum nontoxic concentration (TC0) was 1.00 g/L. In nontoxic concentrations, OjF significantly inhibited HBsAg and HBeAg in 2.2.15 cells after 9 d of treatment (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3705}
}