@article{APS3692,
author = {Ya-bin Jiao and Yao-cheng Rui and Tie-jun Li and Peng-yuan Yang and Yan Qiu},
title = {Expression of pro-inflammatory and anti-inflammatory cytokines in brain of atherosclerotic rats and effects of Ginkgo biloba extract},
journal = {Acta Pharmacologica Sinica},
volume = {26},
number = {7},
year = {2016},
keywords = {},
abstract = {Aim: To study the protein and mRNA expressions of pro-inflammatory and antiinflammatory cytokines in the brain of rats with atherosclerosis (AS) and the effects of Ginkgo biloba extract (GbE) on expressions of cytokines.
Methods: The experimental model of AS in rats were established by intraperitioneal injection of vitamin D3 with high fat/cholesterol diet. GbE 100 mg/kg was administered to rats by ig. After 8 weeks, the expressions of IL-1beta, TNF-alpha, IL-10, and IL-10R in the brain tissues of AS rats were detected by enzyme-linked immunosorbant assay, immunohistochemistry, Western blotting, and reverse transcriptase polymerase chain reaction.
Results: The protein and mRNA expressions of IL-1beta, TNF-alpha, and IL-10 in the brains were markedly higher in AS groups than that in control groups (6.11plusminus0.15, 1.55plusminus0.14, 0.54plusminus0.04 ng/g wet weight vs 0.80plusminus0.14, 0.33plusminus0.09, and 0.33plusminus0.02 ng/g wet weight, respectively). The protein and mRNA expressions of IL-1beta and TNF-alpha in the brains were markedly lower in GbE groups (3.82plusminus0.54, 0.95plusminus0.08 ng/g wet weight) than that in AS groups, the protein and mRNA expressions of IL-10 and IL-10R in the brains were markedly higher in GbE groups (0.85plusminus0.06 ng/g wet weight) than that in AS groups.
Conclusion: GbE inhibited production of pro-inflammatory cytokines IL-1beta and TNF-alpha, but upregulated the production of anti-inflammatory cytokines, IL-10 and IL-10R in brain, which might be related with its anti-AS actions.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3692}
}