@article{APS3620,
author = {Yong-qiu Zheng and Wei Wei and Min Dai and Lei Zhu and Xiao-yi Jia and Yuan Wang},
title = {Interleukin-1 receptor antagonist intervenes in signaling between different types of synoviocytes in rats with adjuvant arthritis},
journal = {Acta Pharmacologica Sinica},
volume = {27},
number = {1},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the mechanisms of interleukin-1 receptor antagonist (IL-1ra)
in the treatment of adjuvant arthritis (AA). Methods: AA was induced in rats by
treatment with Freund’s complete adjuvant (FCA). Rats were given an intracutaneous
injection of IL-1ra (2.5, 10, 40 mg/kg, 3 times per day) from d 14 to d 21 after
immunization. Synoviocyte proliferation and the activity of IL-1 were determined
by using MTT assay. Tumor necrosis factor alpha (TNF-α) and prostaglandin E2
(PGE2) concentrations were measured by radioimmunoassay. The ultrastructure
of synoviocytes was observed by using a transmission electron microscope.
Phosphorylation of c-Jun N-terminal kinase (JNK), extracellular regulating kinase
(ERK) and p38 kinase were detected by Western blot analysis. Results: IL-1ra (10
and 40 mg/kg, ic, d 14–21) modulated the secondary inflammatory reaction
(P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3620}
}