@article{APS3609,
author = {Yun Liu and Wen-lan Sun and Yan Sun and Gang Hu and Guo-xian Ding},
title = {Role of 11-beta-hydroxysteroid dehydrogenase type 1 in differentiation of 3T3-L1 cells and in rats with diet-induced obesity},
journal = {Acta Pharmacologica Sinica},
volume = {27},
number = {5},
year = {2016},
keywords = {},
abstract = {Aim: To observe the roles of 11-β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) in in vitro preadipocyte differentiation and in rats with diet-induced obesity (DIO).
Methods: Protein expression of 11β-HSD1 in the process of 3T3-L1 cell differentiation and in various tissues of the rats were detected by Western blot analysis; expression of 11β-HSD1 mRNA and glucocorticoid receptor (GR) and other marker genes of preadipocyte differentiation were detected by using realtime PCR.
Results: Lipid droplets in 3T3-L1 cells accumulated and increased after stimulation. A dramatically elevated protein level of 11β-HSD1, especially in the late stages of 3T3-L1 cell differentiation, was detected. The relative mRNA levels of 11β-HSD1, GR and cell differentiation markers LPL, aP2, and FAS were upregulated, and Pref-1 was downregulated during the differentiation. In DIO rats, bodyweight, visceral adipose mass index and the protein expression of 11β-HSD1 increased, especially in adipose tissue, brain and muscles. Serum insulin, triglyceride, total cholesterol and low-density lipoprotein cholesterol were found to be increased in DIO rats, but without any obvious changes in blood glucose or tumor necrosis factor-alpha levels.
Conclusion: 11β-HSD1 may promote preadipocyte differentiation, and may be involved in the development of obesity.},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3609}
}