@article{APS3592,
author = {Qing-hui MENG and Li-xin ZHOU and Jia-lin LUO and Jian-ping CAO and Jian TONG and Sai-jun FAN},
title = {Effect of 7-hydroxystaurosporine on glioblastoma cell invasion and migration},
journal = {Acta Pharmacologica Sinica},
volume = {26},
number = {4},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the effect of 7-hydroxystaurosporine (UCN-01), a selective
protein kinase C (PKC) inhibitor, on cell growth, migration, and invasion in invasive
human glioblastoma U-87MG cells. Methods: PKC activity was determined
based on the PKC-catalyzed transfer of the 32P-phosphate group from [g-32P]ATP
into a PKC-specific peptide substrate. Cell viability was measured by MTT assay.
Cell invasion and migration were evaluated by a Boyden chamber assay and
scratch wound assay, respectively. Protein expression was analyzed using Western
blot assay. The formation of 3-dimensional cellular aggregates was examined
by a cell-cell aggregation assay. Results: UCN-01 treatment resulted in concentration-
and time-dependent inhibition of U-87MG cell growth at higher doses (>
100 nmol/L), and reduced cell invasion and migration capability at less cytotoxic
doses (},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3592}
}