@article{APS3590,
author = {Dan-yan ZHU and Yi-jia LOU},
title = {Inducible effects of icariin, icaritin, and desmethylicaritin on directional differentiation of embryonic stem cells into cardiomyocytes in vitro },
journal = {Acta Pharmacologica Sinica},
volume = {26},
number = {4},
year = {2016},
keywords = {},
abstract = {Aim: To investigate the possible inducible effects of icariin, icaritin, and
desmethylicaritin on the directional differentiation of embryonic stem (ES) cells
into cardiomyocytes in vitro. Methods: ES cells were cultivated as embryoid
bodies (EBs) in hanging drops with icariin, icaritin, or desmethylicaritin. ES cells
treated with retinoic acid and with solvent were used as positive and negative
controls, respectively. The cardiomyocytes derived from the ES cells were verified
using immunocytochemistry. The expression of cardiac developmentaldependent
genes was detected using the reverse transcription-polymerase chain
reaction (RT-PCR) method. Cell cycle distribution and apoptosis were analyzed
using flow cytometry to determine the partly inducible effect mechanisms involved.
Results: The total percentage of beating EBs treated with 1×10-7 mol/L icariin,
icaritin, or desmethylicaritin was 87% (P},
issn = {1745-7254}, url = {http://www.chinaphar.com/article/view/3590}
}