TY - JOUR AU - GE Zhi-jun AU - ZENG Yin-ming AU - TAN Yong-fei PY - 2016 TI - Effects of intrathecal 6-hydroxydopamine, α 1 and α 2 adrenergic receptor antagonists on antinociception of propofol in mice JF - Acta Pharmacologica Sinica; Vol 26, No 2 (February 2005): Acta Pharmacologica Sinica Y2 - 2016 KW - N2 - Aim: To investigate the relationship between spinal cord norepinephrine, α 1 and α 2 adrenergic receptors and antinociception of propofol in mice. Methods: Kunming mice were used. Antinociceptive tests were investigated with the tailimmersion test and the acetic acid-induced writhing test. The effects of subcutaneous (sc), intrathecal (ith) and intracerebroventricular (icv) injection propofol on pain threshold were observed. The influences of pretreatment with ith 6- hydroxydopamine, α 1 R antagonist prazosin, or α 2 R antagonist yohimbine on the antinociception of propofol were studied. Results: Significant antinociception was produced by propofol (25, 50 mg/kg, sc) and propofol (20, 40 μg, ith) in tailimmersion test and acetic the acid-induced writhing test ( P P >0.05). The 6-hydroxydopamine (5 and 10 μg), prazosin (5 and 10 μg), or yohimbine (5 and 10 μg) ith alone did not affect basal tai-flick latency (TFL) in conscious mice, but significantly reduced the TFL as measured by tail-immersion test in propofol (50 mg/kg, sc)-treated mice, compared with basal TFL and vehicle groups ( P P Conclusion: The spinal cord is a target of propofol antinociception. In mice propofol antinociception is partly mediated by spinal norepinephrine, α 1 R and α 2 R. UR - http://www.chinaphar.com/article/view/3546