Effects of phencyclidine on rabbit basilar artery in vitro and rabbit cerebral blood flow in vivo

The effect of phencyclidine [1-(1-phenylcyclohexyl)piperidine, PCP] on rabbit basilar arteries was studied with an in vitro model of ring segment arteries. PCP 0.05-500 mumol.L-1 caused vasoconstriction of basilar arteries in a concentration-dependent manner. Its maximal effect (Emax) was 94 +/- 21 mg and the concentration causing half maximal effect (EC50) was 25 +/- 18 mumol.L-1. PCP 0.01-10 mumol.L-1 also concentration-dependently augmented the vasoconstriction induced by electric stimulation in rabbit basilar arteries. Its Emax was 91 +/- 18 mg and EC50 was 0.27 +/- 0.17 mumol.L-1. The effects of PCP on mean arterial blood pressure (MABP) and heart rate (HR) of rabbits were observed. PCP iv 4 mg.kg-1 reduced MABP from 14.3 +/- 0.8 to 12.2 +/- 1.0 kPa and HR from 300 +/- 0 to 278 +/- 5 bpm in 5 min. Using the technique of radionuclide imaging in rabbit brain in vivo, we studied the effect of PCP on cerebral blood flow. After iv PCP 4 mg.kg-1, the tp of radiocerebrogram was increased from 4.5 +/- 1.1 to 6.1 +/- 1.0 s, the tg of radiocerebrogram was increased from 11.7 +/- 0.6 to 18.2 +/- 3.3 s and the rate of clearance was decreased. After iv PCP 2 mg.kg-1, only tg increased from 12.6 +/- 2.1 to 15.9 +/- 0.6 s. Hence PCP increased the transit time of nondiffusible indicators (99mTc) through the cerebral circulation. These results suggest that PCP causes constriction of basilar artery and slows down the cerebral blood flow.