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Acta Pharmacologica Sinica (2009) 30: 435-441; doi: 10.1038/aps.2009.21; published online 23rd March 2009 |
| Original Article | [ Full text ] |
| Sinomenine influences capacity for invasion and migration in activated human monocytic THP-1 cells by inhibiting the expression of MMP-2, MMP-9, and CD147 |
Yang-qiong OU, Li-hua CHEN, Xue-jun LI, Zhi-bin LIN, Wei-dong LI * |
Department of Pharmacology, School of Basic
Medical Sciences,
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Methods: Human monocytic THP-1 cells were induced to differentiate into
macrophages with phorbol 12-myristate 13-acetate
(PMA). Cells were treated with
different concentrations of SIN. The invasion and migration ability of cells was tested by in vitro transwell assays. The levels of CD147 and MMPs were evaluated by flow cytometric analysis and zymographic analysis, respectively. The mRNA expression of CD147, MMP-2, and
MMP-9 was measured by RT-PCR.
Results: The
invasion and migration ability of A-THP-1 cells was significantly inhibited by
SIN in a concentration-dependent fashion; at the same time, the levels of
CD147, MMP-2, and MMP-9 were markedly down-regulated. This inhibitory effect was most notable
at concentrations of 0.25 mmol/L and 1.00 mmol/L (P<0.01).
Conclusion: A possible mechanism of the inhibitory effect of SIN on cell invasion and migration ability is repression of the expression of MMP-2 and MMP-9, which strongly correlates with the inhibition of CD147 activity. |
Keywords: sinomenine; rheumatoid arthritis; invasion; migration; CD147; MMP-2; MMP-9 |
The authors are very grateful to Prof jian-hui LIANG and Dr Michael A McNutt for revising this paper. |
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[ Full text ] |
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