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Acta Pharmacologica Sinica 2008 April; 29 (4): 499-506; doi: 10.1111/j.1745-7254.2008.00765.x |
| Original Article | [ Full text ] |
| Purified polysaccharide from Ginkgo biloba leaves inhibits P-selectin-mediated leucocyte adhesion and inflammation1 |
Rui FEI2,3, Yu FEI4, Sheng ZHENG2, Yan-guang GAO2, Hong-xia SUN5, Xian-lu ZENG2,6 2Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China; 3Department of Cell Biology, School of Norman
Bethune Medical Science, Jilin University, Changchun 130021, China; 4Department of Cardiology, Second Hospital, Jilin University, Changchun
130041, China; 5Department of Thoracic/Head and Neck Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston
Texas 77030, USA |
Methods: The rough PGBL were isolated and purified. The anti-inflammatory effects of purified PGBL (p-PGBL) were assayed by ear edema induced by xylol and the acute peritonitis model in mice. The effect of p-PGBL on inhibiting the interaction between P-selectin and its ligands was investigated by flow cytometry and flow chamber.
Results: p-PGBL could effectively inhibit the acute inflammation in mice and interfere with the adhesion of HL-60 cells, a human leukaemia cell line, or neutrophils to P-selectin in static conditions, as well as the adhesion of neutrophils to Chinese hamster ovary cells expressing human P-selectin and human umbilical vein endothelial cells in flow conditions in a dose-dependant manner.
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Keywords: polysaccharides of Ginkgo biloba leaves; cell adhesion inhibition; inflammation; P-selectin |
| 1 This work was supported by the National Basic Research Program of China (No 2002 CB 513006) and the National Natural Science Foundation of China (No 30570928). |
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