![]() |
Acta Pharmacologica Sinica 2007 March; 28 (3): 439-445; doi: 10.1111/j.1745-7254.2007.00505.x |
| Original Article | [ Full text ] |
| Ceramide induces apoptosis in human lung adenocarcinoma A549 cells through mitogen-activated protein kinases |
Tian-hua ZHANG1,2, Jian-feng LIU3,5, Yao ZHANG2, Yong-li LI2, Hai-tao LU4, Nobuo Murata1, Tatsuo Yamakawa1 1Department of Surgery, Mini-invasive surgery Center, Teikyo University Mizonokuchi Hospital, Kawasaki 213-8507, Japan; 2The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China; 3Department of Neurology, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China; 4Department of Accidents and Emergencies, China-Japan Friendship Hospital, Beijing 100029, China |
Methods: Cell viability and apoptosis were analyzed by cell counting kit-8 assay and flow cytometry. Various MAPK and caspase-3 proteins were detected by Western blotting.
Results: C2-ceramide selectively altered the phosphorylation state of members of the MAPK superfamily, causing hyperphosphorylation of mitogen-activated protein kinase kinase (MEK)1/2 and the p38 MAPK, but not affecting the phosphorylation of extracellular signal-regulated kinase 1/2 and the c-Jun N-terminal kinase. SB-203580 (a p38 MAPK inhibitor) and p38 siRNA, but not U0126 (a MEK inhibitor), partially rescued cell death induced by C2-ceramide. C2-ceramide promoted the activation of caspase-3.
|
Keywords: ceramide; apoptosis; mitogen-activated protein kinases; A549 cells |
|
[ Full text ] |
Copyright©APS 2009 Add: 294 Tai-Yuan Road, Shanghai 200031, China Phn: 86-21-5492-2821 Fax: 86-21-5492-2823 E-mail: aps@mail.shcnc.ac.cn |