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Acta Pharmacologica Sinica 2007 March; 28 (3): 367-374; doi: 10.1111/j.1745-7254.2007.00519.x |
| Original Article | [ Full text ] |
| Endothelial progenitor cell differentiation using cryopreserved, umbilical cord blood-derived mononuclear cells1 |
Jun-ho JANG2,5, Sun-kyung KIM3,5, Jeong-eun CHOI3, Young-jin KIM3, Hyun-woo LEE2, Seok-yun KANG2, Joon-seong PARK2, Jin-hyuk CHOI2, Ho-yeong LIM4, Hugh C KIM2,6 2Department of Hematology-Oncology, Ajou University, School of Medicine, Suwon 442749, Korea; 3Biomedical Research Institute, LifeCord, Suwon 442749, Korea; 4Division of Hematology-Oncology, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135710, Korea |
Methods: MNC from cryopreserved UCB and peripheral blood (PB) were cultured in M199 medium with endothelial cell growth supplements for 14 d. EPC were characterized by RT-PCR, flow cytometry, and immunocytochemistry analysis. The proliferation of differentiated EPC was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay, and vascular endothelial growth factor (VEGF) concentration was measured using an ELISA kit. Characteristics of UCB-derived EPC were compared with those of PB-derived EPC.
Results: A number of round-shaped cells were loosely attached to the bottom after 24 h culture, and numerous spindle-shaped cells began to appear from the round-shaped ones on d 7. Those cells expressed endothelial markers such as, Flt-1/VEGFR-1, ecNOS, VE-cadherin, von Willebrand factor, and secreted VEGF. The patterns of endothelial markers of EPC from PB and UCB did not show striking differences. The results of the proliferation and secretion of VEGF were also similar.
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Keywords: cord blood; endothelial cells; cryopreserved |
| 1 Project supported by grants from the Department of Medical Sciences, The Graduate School, Ajou University, Suwon, Korea. |
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