![]() |
Acta Pharmacologica Sinica 2007 March; 28 (3): 353-358; doi: 10.1111/j.1745-7254.2007.00518.x |
| Original Article | [ Full text ] |
| Biphasic effect of aspirin on apoptosis of bovine vascular endothelial cells and its molecular mechanism |
Qing-quan CHEN, Wen-lan LIU, Xun GUO, Yuan-jian LI, Zhao-gui GUO1 Department of Pharmacology, School of Pharmaceutical Sciences, Central South University, Changsha 410078, China |
Methods: BAEC were cultured and passaged in Dulbecco's modified Eagle's medium culture medium. Morphologic changes and quantification of apoptotic cells were determined using fluorescence microscope after staining the cells with Hoechst 33258. Cell viability was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) method. DNA fragmentation was visualized by agarose gel electrophoresis. Phospho-p38 mitogen-activated protein kinase (MAPK) expression was detected by Western blotting.
Results: Aspirin at low concentrations from 1×10-10 mol/L to 1×10-8 mol/L decreased the apoptosis and p38 MAPK phosphorylation induced by H2O2 in BAEC, while high doses of aspirin (1×10-7-1×10-4 mol/L) induced typical apoptotic changes in BAEC and stimulated the expression of phospho-p38 MAPK in a concentration-dependent manner. SB203580, a specific p38 MAPK inhibitor, blocked such effects.
|
Keywords: aspirin; hydroperoxide; apoptosis; mitogen-activated protein kinase; endothelial cells |
| 1 Project supported by Guangzhou Municipal Key Project Foundation (No 2005Z3E0101) and the Science and Technology Develop-ment Foundation of Guangdong Province (No 2005B34001002 and 2003C34102). |
|
[ Full text ] |
Copyright©APS 2009 Add: 294 Tai-Yuan Road, Shanghai 200031, China Phn: 86-21-5492-2821 Fax: 86-21-5492-2823 E-mail: aps@mail.shcnc.ac.cn |