Acta Pharmacologica Sinica 2007 March; 28 (3): 339-343; doi: 10.1111/j.1745-7254.2007.00520.x

Aim: To investigate whether the increasing CD40-CD40 ligand system is related to restenosis in patients after percutaneous coronary intervention (PCI).

Methods: Twenty normal controls and 120 patients with PCI were investigated. The expression of CD40 and the CD40 ligand (CD40L) on platelets was analyzed by indirect-immunofluorescence flow cytometry. The serum level of soluble CD40L (sCD40L) and C-reactive protein (CRP) was determined by commercially available enzyme linked immunosorbent assay. Restenosis was observed in 120 patients within a 6 month follow-up period after PCI surgery.

Results: Restenosis occurred in 29 patients (24.2%). Patients who developed restenosis showed higher levels of CD40-CD40L system compared with non-restenotic patients before PCI. All restenotic patients showed a significant increase in CD40 [66.9±4.8, mean fluorescence intensity (MFI)] and CD40L (14.9±1.8, MFI) co-expression on platelets as well as sCD40L (13.7±1.7 ng/mL) compared with non-restenotic patients and controls during the 6 month follow-up period (P<0.01). Elevated sCD40L and CD40L were significantly correlated with the serum level of CRP after percutaneous transluminal coronary angioplasty and with lumen loss during the 6 month follow-up period.

Conclusion: The level of CD40L was associated with late restenosis after PCI indicating that restenosis is an inflammatory disorder.

Keywords: CD40; CD40 ligand; restenosis; percutaneous coronary intervention