Acta Pharmacologica Sinica 2007 March; 28 (3): 334-338; doi: 10.1111/j.1745-7254.2007.00497.x

Acta Pharmacologica Sinica 2007 March; 28 (3): 334-338; doi: 10.1111/j.1745-7254.2007.00497.x

Original Article

Upregulation of Mark3 and Rpgrip1 mRNA expression by jujuboside A in mouse hippocampus¹

Cheng WANG, Zi-li YOU², Qing XIA, Tao XIONG, Yang XIA, De-zhong YAO

School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China

Aim: To investigate the effect of jujuboside A (JuA) on modulating gene expression in the hippocampus.

Methods: The spontaneous activity of mice was monitored, and the differential display polymerase chain reaction was adapted to screen differentially-expressed genes modulated by JuA in the mouse hippocampus.

Results: JuA significantly decreased the total activity intensity (P<0.01 vs control) at a dosage of 80 mg/kg, and the genes MAP/microtubule affinity-regulating kinase3 (Mark3) and retinitis pigmentosa GTPase regulator interacting protein1 (Rpgrip1) were upregulated by JuA in the mouse hippocampus.

Conclusion: JuA had an inhibitory effect on the spontaneous activity of the mice, and JuA regulated the transcription of Mark3 and Rpgrip1 in the mouse hippocampus.

Keywords: jujuboside A; hippocampus; DD-PCR; Mark3; Rpgrip1

¹Project supported by a grant from the National Science Fund for Distinguished Young Scholars (No 30525030) and from Key Technologies R&D Program of Sichuan Province.

²Correspondence to Dr Zi-li YOU.
Phn 86-28-8320-0461.
Fax 86-28-8320-1018.
E-mail youzili@uestc.edu.cn
Received 2006-07-08 Accepted 2006-09-22

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