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Acta Pharmacologica Sinica 2007 December; 28 (12): 1947-1956; doi: 10.1111/j.1745-7254.2007.00674.x |
| ORIGINAL ARTICLE | [ Full text ] |
| Astragalus polysaccharide reduces hepatic endoplasmic reticulum stress and restores glucose homeostasis in a diabetic KKAy mouse model1 |
Xian-qing MAO2, Yong WU3, Ke WU2, Ming LIU2, Jing-fang ZHANG4, Feng ZOU2, Jing-ping OU-YANG2,5 2Department of Pathophysiology, Medical College of Wuhan University, Hubei Provincial Key Laboratory of Allergy and Immune-Related Diseases, Wuhan 430071, China; 3Division of Endocrinology and Diabetes, Department of Medicine, University of Oklahoma Health Science Center, Oklahoma city, Oklahoma 73104, USA; 4Department of Pathology and Physiology, Jingmen Vocational College, Jingmen 448000, China |
Methods: The obese and type 2 diabetic KKAy mouse model, which is the yellow offspring of the KK mice expressed Ay gene (700 mg·kg-1·d-1, 8 weeks) and a high glucose-induced HepG2 cell model (200 µg/mL, 24 h) were treated with APS. The oral glucose tolerance test was measured to reflex insulin sensitivity with the calculated homeostasis model assessment (HOMA-IR) index. XBP1 (XhoI site-binding protein 1) transcription and splicing, an indicator of ER stress, was analyzed by RT_PCR and real-time PCR. The expression and activation of glycogen synthase kinase 3 beta (GSK3β), an insulin signaling protein, was measured by Western blotting.
Results: All 4 types of integrins bound soluble fibrinogen in the absence of agonist stimulation, and only the cells expressing the chimeric α subunit with the wild-type β3 subunit, but not those with truncated β3, could adhere to and spread on immobilized fibrinogen.
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Keywords: Astragalus membranaceus; polysaccharide; type 2 diabetes mellitus; insulin resistance; endoplasmic reticulum; stress |
| 1 Project supported by grants from the National Natural Science Foundation of China (No 30370673). |
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