![]() |
Acta Pharmacologica Sinica 2007 January; 28 (1): 19-27; doi: 10.1111/j.1745-7254.2007.00469.x |
| Original Article | [
Full text] |
| Neuroprotection of selenite against ischemic brain injury through negatively regulating early activation of ASK1/JNK cascade via activation of PI3K/AKT pathway |
Qing WANG, Quan-guang ZHANG, Dong-na WU, Xiao-hui YIN, Guang-yi ZHANG2 Research Center for Biochemistry and Molecular Biology, Xuzhou Medical College, Xuzhou 221002, China |
Methods: Transient global brain ischemia was induced by 4-vessel occlusion into adult male Sprague-Dawley rats weighing 250_300 g. The rats were pretreated only with selenite (0.3 mg/kg dissolved in 0.9% saline) every 24 h for 7 d by means of intravenous injection of the tail or combined with LY294002 from d 5 by left cerebral ventricle injection before surgery.
Results: Selenite significantly increased AKT1 activation and decreased the activation of ASK1/JNK cascade via phosphorylating ASK1 at Ser-83 residue by AKT1 during early reperfusion after 15 min transient global brain ischemia. On the contrary, combined pretreatment of the rats with LY294002 (a specific PI3K inhibitor) and selenite significantly inhibited the effects solely with selenite.
|
Keywords: cerebral ischemia; PI3K/AKT; ASK1/JNK; LY294002; selenite |
| 1 Project supported by a grant from the key project of the National Natural Science Foundation of China (No 30330190). |
|
[
Full text ] |
Copyright©APS 2009 Add: 294 Tai-Yuan Road, Shanghai 200031, China Phn: 86-21-5492-2821 Fax: 86-21-5492-2823 E-mail: aps@mail.shcnc.ac.cn |