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Acta Pharmacologica Sinica 2007 August; 28 (8): 1175-1180; doi: 10.1111/j.1745-7254.2007.00566.x |
| Original Article | [ Full text ] |
| Propofol attenuation of renal ischemia/reperfusion injury involves heme oxygenase-1 |
Hui-hua WANG, Hai-yan ZHOU1,3, Cong-cong CHEN1, Xiu-lai ZHANG1, Gang CHENG2 Departments of 1Anesthesiology and 2Cardiology, the Second Affiliated Hospital, Zhejiang University Medical School, Hangzhou 310009, China |
Methods: Sprague-Dawley rats were randomly divided into 3 groups: (i) sham-operated group; (ii) I/R group; and (iii) propofol group. Bilateral renal warm ischemia for 45 min was performed. After 2, 6, and 24 h reperfusion, blood samples and kidneys were collected for assessment of renal injury, and HO-1 expressions were analyzed by immunohistochemical analysis, RT-PCR and Western blotting.
Results: Blood urea nitrogen and serum creatinine levels in the propofol group were significantly lower than that in the I/R group at 24 h after reperfusion. The mean histological score by Paller's standard showed that propofol significantly attenuated renal I/R injury after 6 h reperfusion. Propofol increased HO-1 mRNA and protein levels 2 h after reperfusion, whereas HO-1 expressions were present at exceedingly low levels in the I/R group and the sham-operated group at same time point. Propofol also markedly increased HO-1 mRNA and protein levels than I/R at 6 and 24 h after reperfusion.
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Keywords: propofol; reperfusion injury; heme oxygenase-1; kidneys |
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[ Full text ] |
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