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Acta Pharmacologica Sinica 2007 August; 28 (8): 1116-1122; doi: 10.1111/j.1745-7254.2007.00598.x |
| Original Article | [ Full text ] |
| Tanshinone IIA inhibits endothelin-1 production in TNF-α-induced brain microvascular endothelial cells through suppression of endothelin-converting enzyme-1 synthesis |
Chao TANG1, An-hua WU1,3, Hong-li XUE2, Yun-jie WANG1 1Department of Neurosurgery, the First Affiliated Hospital, China Medical University; 2Department of Neurosurgery, General Hospital of Shenyang Military Command, Shenyang 110001,China |
Methods: The ET-1 release (including large ET-1) into the culture medium was determined by enzyme immunoassay. The levels of ET-1, ECE-1, ETA, and ETB mRNA were measured by RT-PCR. Endothelin receptor binding was also tested.
Results: The induction of ET-1 release by TNF-α from cultured BMVEC was dose-dependently reduced by Tan IIA, but large ET-1 levels progressively increased in response to Tan IIA; the mRNA expression of ET-1 was unaffected. Tan IIA also caused a decrease in ETA receptor mRNA and ECE-1 expression in a dose-dependent manner. Endothelin receptor binding was unaltered in BMVEC stimulated with TNF-α alone or a combination of TNF-α and Tan IIA.
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Keywords: tanshinone IIA; endothelin-1; TNF-α; brain microvascular endothelial cells |
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