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Acta Pharmacologica Sinica 2006 June; 27 (6): 659-664; doi: 10.1111/j.1745-7254.2006.00324.x |
| Original Article | [ Full text ] |
| Inhibition of ethanol-induced toxicity by tanshinone IIA in PC12 cells1 |
Xian-fang MENG2, Xiao-jing ZOU3, Bin PENG2, Jing SHI2, Xin-min GUAN2, Chun ZHANG4,5 2Department of Neurobiology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; 3Department of Anesthesiology, 4Department of Nephrology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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Aim: To observe the effects of tanshinone IIA (Tan IIA) on the neurotoxicity induced by ethanol in PC12 cells and to explore its protective role.
Methods: PC12 cell survival was measured by MTT assay. The formation of reactive oxygen species (ROS) and lactate dehydrogenase (LDH) release were detected by 2',7'-dichlorofluorescin (DCF) fluorescence and calorimetric method, respectively. The percentage of cell apoptosis was monitored by flow cytometry. The expression of p53 was detected by immuno-fluorescence and flow cytometry.
Results: Ethanol significantly impaired the survival of PC12 cells as demonstrated by MTT assay. Ethanol also induced significant ROS formation and increased LDH release. Pre-incubation with Tan IIA in the culture medium significantly reversed these changes. Ethanol caused cell apoptosis and the upregulation of p53 protein. The anti-apoptosis effects of Tan IIA on ethanol-induced toxicity were accompanied by the downregulation of pro-apoptotic p53 protein expression.
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Keywords: apoptosis; ethanol; PC12 cells; p53; tanshi-none IIA |
| 1 Project supported by a grant from the National Natural Science Foundation of China (No 30400122). |
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