Acta Pharmacologica Sinica 2006 April; 27 (4): 485-490; doi: 10.1111/j.1745-7254.2006.00284.x

 
Original Article
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Deguelin inhibits expression of IkBα protein in Raji and U937 cells1
 

Wei-hua CHEN, Yan CHEN2, Guo-hui CUI

Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China

 

Aim: To determine whether deguelin can regulate the expression of nuclear factor kappa B (NF-kB) binding protein (IkBα) in U937 human leukemia cells and Raji human B lymphoma cells.

 

Methods: The localization of IkBα protein was investigated by using an immunofluorescence method. The expression of IkBα and NF-kB /p65 proteins in Raji and U937 cells were investigated by using Western blotting. Apoptosis was detected through annexin V/PI double-labeled cytometry.

 

Results: IkBα localized in the cytoplasm in untreated and deguelin-treated cells. After treatment with tumor necrosis factor a (TNF-α) or deguelin plus TNF-α for 15 min, there was a substantial reduction in the amount of IkBα protein. The expression of IkBα was downregulated by deguelin in Raji and U937 cells. Deguelin induced apoptosis in U937 cells.


Conclusion:
Deguelin inhibited the expression of IkBa protein in U937 and Raji cells. The anti-proliferative activity of deguelin is related to the signal pathway of NF-kB.

 

 

Keywords: deguelin; IkBα; nuclear factor kappa B; tumor necrosis factor-α; apoptosis; Raji cells; U937 cells

 

1 Project supported by the National Natural Science Foundation of China (No 30472267).
2 Correspondence to Prof Yan CHEN.
Phn 86-27-8572-6387.
Fax 86-27-8572-6916.
E-mail yanchen@public.wh.hb.cn
Received 2005-09-19     Accepted 2005-10-25

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