![]() |
Acta Pharmacologica Sinica 2006 April; 27 (4): 423-429; doi: 10.1111/j.1745-7254.2006.00296.x |
| Original Article | [ Full text ] |
| All-trans retinoic acid inhibited angiotensin II-induced increase in cell growth and collagen secretion of neonatal cardiac fibroblasts1 |
Yan HE2, Ying HUANG2, Li ZHOU2, Li-min LU2, Yi-chun ZHU2, Tai YAO2,3,4 2Department of Physiology and Pathophysiology, and 3State Key Laboratory for Medical Neurobiology, Fudan University Shanghai Medical College, Shanghai 200032, China |
|
Aim: To determine whether all-trans retinoic acid (atRA) acts to modulate angiotensin II (Ang II)-induced cardiac fibroblast cell growth and collagen secretion. Methods: Cultured neonatal rat cardiac fibroblasts (CF) were used in the experiment. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to detect cell growth of the CF; and immunocytochemistry and Western blotting were used to measure the production and secretion of collagen and the expression of transforming growth factor-β1 (TGF-β1) by the CF. Results: atRA (1×10-7 to 1×10-5 mol/L) inhibited the Ang II-induced increase in cell growth of CF (P<0.05). Ang II stimulated the secretion of collagen types I and III by the CF. This effect was blocked by AT1 receptor antagonist losartan (1×10-6 mol/L), but not by AT2 receptor antagonist PD123319 (up to 1×10-6 mol/L). Exposure of CF to atRA (1×10-5 mol/L) attenuated the Ang II-induced increase in the secretion of collagen types I and III (P<0.05). atRA (1×10-5 mol/L) also blocked the Ang II-induced increase in the expression of TGF-β1. Conclusion: atRA inhibits the Ang II-induced increase in cell growth and collagen secretion of neonatal rat CF. The effect of atRA is possibly mediated by lowering the TGF-β1 level. These observations support the notion that atRA is a potential candidate for the prevention and therapy of cardiac remodeling. |
Keywords: cardiac fibroblast; angiotensin II; all-trans retinoic acid; collagen secretion; transforming growth factor-β1 |
|
[ Full text ] |
Copyright©APS 2009 Add: 294 Tai-Yuan Road, Shanghai 200031, China Phn: 86-21-5492-2821 Fax: 86-21-5492-2823 E-mail: aps@mail.shcnc.ac.cn |