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Acta Pharmacologica Sinica 2006 December; 27 (12): 1526-1536; doi: 10.1111/j.1745-7254.2006.00438.x |
| ORIGINAL ARTICLE | [ Full text ] |
| Cysteinyl leukotriene receptor 1 is involved in N-methyl-D-aspartate-mediated neuronal injury in mice |
Qian DING, Er-qing WEI2, Yan-jun ZHANG, Wei-ping ZHANG, Zhong CHEN Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou 310058, China |
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Aim: To determine whether cysteinyl leukotriene receptor 1 (CysLT1 receptor) is involved in N-methyl-D-aspartate (NMDA)-induced excitotoxic injury in the mouse brain.
Methods: Brain injury was induced by NMDA microinjection (50-150 nmol in 0.5 µL) into the cerebral cortex. The changes in CysLT1 receptor expression 24 h after NMDA injection and the effects of a CysLT1 receptor antagonist, pranlukast (0.01 and 0.1 mg/kg), an NMDA receptor antagonist, ketamine (30 mg/kg), and an antioxidant, edaravone (9 mg/kg) were observed.
Results: In the NMDA-injured brain, the CysLT1 receptor mRNA, and protein expression were upregulated, and the receptor was mainly localized in the neurons and not in the astrocytes. Pranlukast, ketamine and edaravone decreased NMDA-induced injury; pranlukast (0.1 mg/kg) and ketamine inhibited the upregulated expression of the CysLT1 receptor.
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Keywords: cysteinyl leukotriene receptor; excitotoxi-city; N-methyl-D-aspartate; leukotriene receptor antagonist; pranlukast; NMDA receptor antagonist; ketamine |
| 1 Project supported by the National Natural Science Foundation of China (No 30371637). |
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