Acta Pharmacologica Sinica 2006 September; 27 (9): 1119-1126; doi: 10.1111/j.1745-7254.2006.00362.x

 
Original Article
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Microtubule stability and MAP1B upregulation control neuritogenesis in CAD cells
 

Wen LI2,5, Jin-tang XIA3, Yue FENG4,5

2Laboratory of Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, China; 3Department of General Surgery, The First People's Hospital of Guangzhou, Guangzhou 510180, China; 4Department of Pharmacology, Emory University School of Medicine,USA

 

Aim: To study the role of microtubule dynamics and microtubule associated protein 1B (MAP1B) in regulation of the neurite extension in CAD catecholaminergic neuronal cell line.

 

Methods: The neuritogenesis of the CAD cells was abolished by inhibiting microtubule polymerization with nocodazole and by blocking microtubule depolymerization with taxol. MAP1B and tubulin protein expression was detected by Western blot. Immunofluorescent staining of tubulins was observed by fluorescent and confocal microscopy.

 

Results: Microtubule dynamics was essential for CAD neurite extension. Dosage analysis revealed that neurite extension was much more sensitive to nocodazole than to taxol, suggesting a functional requirement for highly active microtubule assembly. A remarkable upregulation of MAP1B protein was detected during neurite extension accompanied with increased microtubule stability.


Conclusion:
Upregulation of MAP1B leads to the stabilization of newly formed microtubules in the developing neurites, which in turn promotes neurite extension.

 

Keywords: microtubule stability; microtubule associated protein 1B; neuritogenesis

 
1Project partly supported by NIH grant 5 PO1 HD35576 (Project III) to YF and to PG and a grant from Guangdong Science Foundation (No 5001776).

5Correspondence to Dr Wen LI and Yue FENG.
Phn 86-20-8775-5766, ext 8252.
E-mail wenli28@163.com
Received 2006-01-07     Accepted 2006-03-01

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